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Figure 1
Validation of cross RF peaks using the SRF in the case of an oligomer search model. (a) The χ = 180° section of a native SRF indicates 222 molecular symmetry. (b, c) χ = 180° sections of an SRF in P1 generated using Fcalc from a tetrameric search model in orientations corresponding to the fourth (correct) and eighth (incorrect) highest peaks of the cross RF. The consistency of the peaks in (a) and (b) suggest that the fourth cross RF peak is likely to be a correct cross RF solution. This figure was generated using MOLREP (Vagin & Teplyakov, 1997BB53), Proteus vulgaris tryptophanase data (PDB code 1ax4 ; Isupov et al., 1998BB20) and the Citrobacter intermedius tetrameric tyrosine phenol-lyase model (PDB code 1tpl ; Antson et al., 1993BB2).

Journal logoBIOLOGICAL
ISSN: 1399-0047
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