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Figure 2
Structure solution of DKMO (a) The superposition of β2 and αβ dimers of bacterial luciferase from V. harveyi indicates conservation of a dimer. (b) Bottom, χ = 180° section of the SRF defining the orientation of the NCS dyad in the DKMO structure; top, reference orientations of the search model (synthetic α2 dimer) in which the molecular dyads are aligned with the NCS twofold axis (blue lines). (c) TF searches were performed for both reference dimers rotated around the twofold axis by the variable angle χ. The highest correlation coefficient (CC) in the TF search was plotted as a function of χ. The highest peak at 165° in the top plot corresponds to the correct orientation. (d) 2FoFc (blue) and FoFc (red) electron-density maps after REFMAC refinement of the MR model (colored by atom type) with DMMULTI external phases superposed on the final refined structure of DKMO (green). (e) Ribbon representation of the DKMO dimer. This figure was prepared using BOBSCRIPT (Esnouf, 1997BB14), MOLREP and R (R Development Core Team, 2005BB40).

Journal logoBIOLOGICAL
ISSN: 1399-0047
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