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Figure 3
Crystal/beam relationship. (a) Large and small crystals used for data collection with dimensions of 180 × 180 × 30 and 20 × 20 × 70 µm, respectively; 1 µm beam spot. (b) Strategy for data acquisition. After every rotation, new material is present in the narrow beam channel. A small intentional misset (2–5 µm) of the rotation axis was introduced. During data collection this creates a cylinder around the rotation centre, with the multiply illuminated area lying on the surface of the cylinder. Consequently, this minimizes the inclusion of damaged crystal volume contributing to individual diffraction patterns during total exposure.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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