 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](be5118fig3mag.jpg)
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Figure 3
X-ray structures of EmrE. (a) The non-native structure of EmrE determined from crystals grown at pH 4.5 viewed parallel to the membrane plane with the positions of Glu14 shown as space-filling models and (b) the structure of one of the monomers viewed perpendicular to the membrane plane in rainbow coloration (N-terminus blue, C-terminus red); the structures are from PDB entry 1s7b , which has the same overall structure as the revised 3b61 . (c) The corrected X-ray structure of EmrE containing bound TPP+ viewed parallel to the membrane plane (PDB code 3b5d ). Electron density corresponding to Se from MAD data is shown as either a red mesh or green mesh depending upon the monomer in which the SeMet residues reside (numbered). The density corresponding to As in the tetraphenylarsonium substrate is shown as a purple mesh. (d) Comparison between the model based upon the cryo-EM structure and evolutionary constraints and the corrected X-ray structure. (c) and (d) are reprinted with permission from Chen et al. (2007  ), (Copyright 2007, National Academy of Sciences, USA). |
![[Figure 3]](be5118fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
