 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 2]](be5126fig2mag.jpg)
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Figure 2
Monovalent cation-dependence of the DNA strand-exchange activity of MmRadA. The strand reaction scheme is shown in (a). The reaction solutions contained 2 mM of a specified nucleotide, 100 mM of a specified monovalent salt, 10 mM MgCl2 and 50 mM HEPES–Tris buffer pH 7.4. The solutions also contained 15 µM MmRadA and 1 µM each of ssDNA (FAM-labelled) and dsDNA (unlabelled) substrates. Strand-exchange activity was indicated by the formation of the slowest migrating heteroduplex DNA species (FAM-labelled hdDNA). The intrinsic fluorescence from the FAM label was recorded as shown in (b) and (c). |
![[Figure 2]](be5126fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
