Figure 3
Crystal structure of MmRadA in complex with AMPPNP in stereo. (a) OMIT (Fo − Fc) difference Fourier map at 2.0 Å resolution. The AMPPNP molecule is shown as a stick model. The possible locations of two ammonium ions are shown as cyan spheres. (b) Two conformational forms of MmRadA. Two AMPPNP molecules and the side chains of His280 are shown as stick models. The protein is shown in ribbon representation. The DNA-interacting loops are highlighted in cyan for the recurrent inactive form observed in NaCl, RbCl or a low concentration (<0.3 M) of KCl or NH4Cl. They are highlighted in magenta for the ATPase-active form observed in 0.4 M KCl or NH4Cl. |