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Figure 2
Protein crystallization in COP cards. (a) Lysozyme and trypsin crystals formed in COP cards using the microbatch protocol. (b) Crystallization of human p53–DNA complexes in COP cards using the microbatch and vapour-diffusion protocols and three precipitant solutions (Index Screen Nos. 87, 89 and 90). Each condition was performed in triplicate or quadriplicate (numbered 1–3 and 1–4, respectively) and the results are colour-coded as follows: protein precipitate, grey; protein crystals, purple; clear solution, white. Examples of the crystals that were formed using each protocol are shown. (c) Crystallization of A. thaliana MOM1 in COP cards using the microbatch, vapour-diffusion and free-interface diffusion protocols. Each condition was performed in quadriplicate (numbered 1–4) using magnesium formate as the precipitant at the indicated concentrations. The results are colour-coded as described for the p53–DNA complexes in (b). For the free-interface diffusion protocol, both the protein (left half) and the precipitant (right half) chambers were scored, since over time both chambers will contain both protein and precipitant. Examples of the crystals formed using each protocol are shown. All images were acquired using the camera built into the microfluidics instrument. The width of the chambers is 750 µm. The detailed compositions of the precipitant solutions are described in §[link]2.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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