Figure 3
Collection of X-ray diffraction data sets from crystals in COP cards. (a) X-ray diffraction pattern of a p53–DNA crystal exposed to the X-ray beam while still in the COP card. The oscillation range was 1°. (b) X-ray diffraction patterns of a lysozyme crystal exposed to the X-ray beam while still in the COP card. Two regions of the diffraction image are shown, one encompassing a resolution range lower then 3.5 Å (left) and the other a region from 1.9 to 1.6 Å resolution (right). The oscillation range was 1°. Note that COP absorbs X-rays in the resolution range between 5.4 and 5.1 Å. (c) Part of the lysozyme electron-density map contoured at 1.9σ for the 2Fo − Fc map (olive green) and at 3σ for the Fo − Fc map (dark blue, positive values; orange, negative value). The map shows residues Trp46, Val47 and Ile116 and part of the side chain of Trp126. |