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![[Figure 6]](ba5140fig6mag.jpg)
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Figure 6
(a) Structure of the Br-DIS molecule. Br-DIS is a 23-nucleotide RNA fragment corresponding to the dimerization-initiation site (DIS) of HIV-1(Lai) genomic RNA, with 5-bromouridine substituted for uridine at position 3 (Ennifar et al., 2002  ). (b) X-ray-induced decay of the Br atoms. The occupancy factors of the Br atoms were refined against unmerged data by applying an exponential decay model [equation (9); Schiltz et al., 2004]. The rest of the structure remained remarkably stable during the X-ray data collection. This was therefore an almost ideal example of a case in which the substructure (H), here consisting of the two Br atoms, undergoes a continuous change during the X-ray data collection, whereas the remainder of the structure (P) remains nearly unchanged. The differential modulation of H and P gave rise to measurable intensity differences between symmetry-related reflections that were measured at different X-ray doses. |
![[Figure 6]](ba5140fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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