Figure 7
Phasing from unmerged data in the case of Br-DIS (Schiltz et al., 2004). Views of the electron-density maps obtained for Br-DIS to 2.46 Å resolution after phasing with SHARP and solvent flattening. (a) Standard SAD phasing on a merged data set. (b) A substantial improvement of the SAD phases was obtained by using unmerged data and modelling the X-ray-induced decay of the Br sites. The refined structural model of the Br-DIS molecule is superimposed on the maps. |