Figure 2
Electron-density maps calculated at various stages of the fH678 structure solution with the final coordinates displayed as stick models. The inset shows a close-up of a typical portion of the map in a region for which no molecular model existed at the point of calculating the map. (a) A SOLOMON solvent-flattened map derived from SHARP phase distributions generated from SeMet anomalous data. (b) BUSTER-TNT phases generated by refining fH7 against the fH678 data with missing atoms modelled. (c) SOLOMON solvent-flattened map using SHARP phase distributions generated from SeMet anomalous data in which BUSTER-TNT phase distributions (as in b) were used within the SHARP refinement and for calculation of SHARP output phase distributions. |