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![[Figure 6]](ba5142fig6mag.jpg)
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Figure 6
Phasing in both hands. The anomalous scattering component is always advanced. For example, data collected at a wavelength of 1.7 Å from an iron-containing protein will have a significant anomalous signal from both the Fe atoms and the S atoms in methionine and cysteine. Non-anomalous contributions to the scattering come from C, N and O atoms. The total structure factor has an anomalous component that is not perpendicular to the normal scattering component, leading to an anomalous difference in the structure factors for F+ and F−. Only in one hand will the observed direction of the anomalous difference match the calculated direction of the difference (|F+| > |F−|). |
![[Figure 6]](ba5142fig6.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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