Figure 1
(a) Wilson plots (average observed J in resolution shells) for P19–siRNA data. Black and grey squares correspond to the fresh crystal and to the crystal after an absorbed dose of 30 MGy, respectively. The BEST-predicted Wilson plots, i.e. the average values of calculated for the same set of reflections, are represented by solid and dashed lines, respectively. (b) Relative scale and B factors as a function of radiation dose. Isotropic B-factor scaling to a common reference scale is performed by BEST. The scale factors are divided by those of the first data set and the B factor of the first data set is subtracted from the B factors of subsequent data sets. |