Figure 5
IDE for native (solid lines) and SeMet-derivative (broken lines; ten Se atoms measured by microPIXE; Garman & Grime, 2005) crystals of the protein PPK of various sizes. For a given crystal size, the value of IDE was normalized to unity at the peak value for the native form of the crystal. All calculations shown here were performed assuming a top-hat-shaped beam of size equal to that of the crystal with a photon flux of 1012 photons s−1 and an exposure time of 0.2 s per image and took into account both X-ray fluorescent escape and Compton scattering. It is clear that diffraction-dose efficiency (IDE) reaches a peak for crystals of all sizes at photon energies of 24–34 keV. |