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![[Figure 3]](ba5148fig3mag.jpg)
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Figure 3
Radiation-damage model. The observations made by Owen et al. (2006  ) and Kmetko et al. (2006) are reproduced with permission from the original publishers and plotted against predicted curves derived from two alternative radiation-damage models. The `H model' is an exponential decay of spot intensity with dose and the `B model' is the dose-dependent B-factor model suggested by Kmetko et al. (2006). The `H model' predictions were made by applying (13) to intensities derived from the observed structure-factor file deposited with the indicated PDB entry and then computing the sum of all intensities (a) followed by scaling the `simulated damage' intensities to the `zero-dose' intensities (b) using the procedure described by Kmetko et al. (2006). The `B model' prediction curves (dotted lines) were prepared similarly except that the `simulated damage' intensities were generated by applying the relevant dose-dependent B factor reported by Kmetko et al. (2006). All `H model' curves (solid lines) used the same value of H (10 MGy Å−1) and therefore may explain the dissimilar `sensitivity parameter' observed by Kmetko et al. (2006) for apoferritin and lysozyme (orange circles versus blue squares, respectively). It is clear from (a) that the `B model' is at odds with the observations of Owen et al. (2006) (green diamonds), although the same predicted intensities are in very good agreement with the data points from Kmetko et al. (2006) (orange circles). Agreement between these two studies is restored, however, if we accept the `H model' where the resolution-dependence of radiation damage is exponential as opposed to a Gaussian (B model). |
![[Figure 3]](ba5148fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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