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Figure 3
The process of constraining PDB entry 3b77 into a tetragonal space group, P4, starting with a model (structure A2) that is intentionally solved in space group P2. The monoclinic asymmetric unit contains 12 polypeptide chains (labeled AL). The twofold operator 2 of space group P2 generates a second asymmetric unit populated by chains A′–L′. The trial fourfold (marked by `?') maps chains AF to chains GL and maps chains GL to chains A′–F′.

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