Figure 1
(a) Microcrystals of H2L6 (28% PEG 8000, 0.1 M MES pH 6.5) used for MMS. (b–e) H2L6 crystals after the first round of MMS obtained under the following conditions: (b) 20% PEG 3350, 0.2 M lithium acetate pH 7.9, (c) 20% PEG 3350, 0.2 M ammonium tartrate pH 6.6, (d) 20% PEG 3350, 0.2 M ammonium phosphate pH 8.0, (e) 20% PEG 3350, 0.2 M ammonium citrate pH 5.1. (f, g) Diffraction-quality H2L6 crystals after the second round of MMS obtained under the following conditions: (f) 14% PEG 3350, 0.2 M ammonium tartrate, 0.1 M MES pH 6.5, (g) 16% PEG 3350, 0.2 M ammonium citrate, 0.1 M MES pH 6.5. Scale bars are 0.3 mm in length. |