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Figure 3
Crystals of C836. (a, b) Microcrystals used to generate a self-seed stock obtained from the following conditions: (a) 25% PEG 8000, 0.1 M HEPES pH 7.5, (b) 20% PEG 3350, 0.2 M lithium citrate pH 8.4. (c–f) Diffraction-quality crystals of C836 obtained by MMS under the following conditions (all contain 0.1 M HEPES pH 7.5): (c) 18% PEG 3350, 0.2 M ammonium citrate, self-seeds, (d) 20% PEG 3350, 0.2 M ammonium citrate, cross-seeds, (e) 22% PEG 3350, sodium formate, cross-seeds, (f) 20% PEG 3350, 0.2 M sodium tartrate, cross-seeds. Scale bars are 0.2 mm in length. |