Figure 4
Nanoflow ESI-MS of enolase and RNase E microdomain (823–850) under nondissociating conditions. (a) ESI mass spectrum with a mass corresponding to that of an enolase dimer bound to one molecule of RNase E microdomain (actual mass 94 268 Da, theoretical mass 94 083 Da). (b) Tandem mass spectrum of the +19 species in (a), showing the presence of the RNase E microdomain (actual mass 3000 Da, theoretical mass 3036 Da). |