Figure 1
SANS differential cross-sections of Ncd281 microtubules in fD = 0.42 D2O with AMPPNP and comparison with models. Ncd281 data are shown as circles. Total particle densities for Ncd281: N = 3.92 × 1016 cm−3 motor dimers; N = 5.89 × 1016 cm−3 tubulin dimers. Scattering intensity calculated for models: (a) one-head cooperative binding; (b) one-head random binding with an average of approximately one motor per helical turn; (c) two-head cooperative binding. In each of the cooperative binding models sections of decorated microtubules coexist with sections of undecorated microtubules. Model parameters are given in Table 2. All values are per tubulin dimer, dσT(Q)/dΩ (cm2), scaled and corrected for the fraction of unbound Ncd as described in the text. |