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Figure 3
Solubility data in PEG 6000 for selected mutants of RhoGDI (Cooper et al., 2007BB8) plotted on a logarithmic scale following Cohn's equation, logcp = BKc, where cp is the protein concentration, c is the precipitant concentration, B is the idealized protein solubility at c = 0 and K is a protein-dependent constant. For all mutants, the solubility in PEG 6000 is systematically lowered and follows the pattern observed by Trevino et al. (2007BB35). Importantly, the K parameter appears unchanged, so that the solubility lines are approximately parallel. The replacement of lysines by alanines causes only a marginal decrease in solubility; the effect is more significant when threonines are introduced and is the largest with tyrosines. Replacing glutamates by alanines causes a significantly larger solubility reduction. Solubility was measured at room temperature at pH 8.0 (buffered) as a function of PEG 6000 concentration.

Journal logoBIOLOGICAL
ISSN: 1399-0047
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