view article

Figure 3
X-ray background reduction obtained by precisely matching X-ray beam and crystal size. (a) AcMNPV polyhedra crystals of approximate dimensions 5 × 5 × 5 µm were mounted on a Micromesh grid. The enlarged area illustrates the FWHM extent of the two different beam sizes used for this test aligned around one of the polyhedra crystals. The Gaussian profiles shown are for illustration purposes only. (b) Identical regions of diffraction images from the crystal in (a) taken using an 8 × 8 µm beam for 1 s and a 4.5 × 5 µm beam for 3.3 s. Exposure times were adjusted so that the average integrated intensity from both images was similar. The images were displayed with ADXV using identical contrast levels. The inserts show pixel values in the region of the same diffraction spot (−12 −3 17), where a reduction in average background of about 1/3 is observed. This reflection was chosen because it was a fully recorded reflection of significant intensity as indicated by MOSFLM.

Journal logoBIOLOGICAL
ISSN: 1399-0047
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds