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![[Figure 5]](yt5033fig5mag.jpg)
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Figure 5
DNA-replication activity of T-ag mutant proteins. (a) Transient DNA-replication activities of the indicated wt or mutant T-ag proteins in C33A cells. DNA-replication activities were measured by determining the ratio of firefly (Fluc-ori plasmid) to Renilla (Rluc control plasmid) luciferase activities as described in § ![[link]](../../../../../../logos/arrows/d_arr.gif) 2. Replication activities are reported as a percentage of the Fluc:Rluc ratio obtained with the largest amount of wt T-ag expression vector (12 ng). Cells transfected with vector only (No T-ag) were used as a negative control. (b) Expression of T-ag proteins. Western blot analysis of total protein extracts prepared from transfected C33A cells expressing the wt or the indicated T-ag mutant proteins. Each protein was tested using a gradient of expression vector (0, 1.25, 2.5, 5.0, 10 and 12.5 ng). 100% replication was defined as the amount of replication observed using 12.5 ng wt T-ag. |
![[Figure 5]](yt5033fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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