 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 8]](mh5057fig8mag.jpg)
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Figure 8
Generation of a new cleft at the bottom of the groove and water penetration into the cleft at 580 MPa. (a) Pro108 and Leu305 at the bottom of the groove on the opposite side to the active site at a series of pressures. The main chains are shown as cartoon representations (white, 0.1 MPa; yellow, 340 MPa; orange, 410 MPa; pink, 580 MPa; magenta, 650 MPa). Pro108 and Leu305 are represented as wires (red, O atoms; blue, N atoms). (b, c) Difference electron-density map between Pro108 and Leu305 shown as a green mesh contoured at 3.0σ at 0.1 and 580 MPa, respectively. Pro108, Ser266 and Leu305 are represented as sticks. Three positive peaks are observed at 580 MPa in (c) and were assigned as three water molecules: W697, W698 and W699 (represented by red balls). No positive peaks are observed in the cleft at 0.1 MPa in (b). (d) Solvent-excluded surface representation around Pro108 and Leu305 at 580 MPa, drawn using a probe radius of 1.4 Å. (e) Hydrogen-bond network of the penetrating water molecules W697, W698 and W699 as illustrated by yellow dashed lines |
![[Figure 8]](mh5057fig8.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
