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![[Figure 1]](kw5044fig1mag.jpg)
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Figure 1
Application of morphing to the cab55348 structure. (a) Cip2 template (Pokkuluri et al., 2011  ) in blue; final model of cab55348 in green; prime-and-switch electron-density map based on the template structure in purple. (b) Cip2 template and map as in (a); template after refinement with phenix.refine in orange. (c) Cip2 template and map as in (a); model density calculated from Cip2 template in purple. (d) Cip2 map as in (a); model density calculated from Cip2 template, offset to optimally match map, in purple. (e) Cip2 template and map as in (a); morphed Cip2 model in yellow. (f) Cip2 template, map and morphed Cip2 model as in (e); refined morphed Cip2 model in off-yellow. (g) Refined model after six cycles of morphing in yellow; prime-and-switch map based on model from cycle 5 of morphing in purple. (h) Automatically rebuilt model in green and density-modified electron density map in blue obtained starting from the map and model in (g). Contour levels in all the maps are at 1.5σ except for the model densities in (c) and (d), which are at 3.5σ. Figures were created with Coot (Emsley et al., 2010) and Raster3D (Merritt & Bacon, 1997). A full Coot scene with all models and maps is available as supplementary material. |
![[Figure 1]](kw5044fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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