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Figure 5
Change in UV–Vis absorbance of FcγRIIIa crystallization solution at 240 and 593 nm irradiated at 100 K and then warmed to 180 K. The x axis reflects the readback temperature from the cryojet controller; the temperature at the sample will differ from this. Note that this axis is nonlinear and approximate. At approximately 150 K absorbance at low wavelengths decreases significantly, indicating increased mobility of radicals. The absorbance at 240 K does not return to zero owing to discolouration and icing of the sample upon slow warming.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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