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Figure 1
(a) The primary structure of WbdD. The domain borders were placed according to the crystal structure of WbdD556. (b) SDS–PAGE of WbdD600 samples from limited proteolysis reactions. Lane M contains NuPAGE Mark12 protein marker (Invitrogen). The molar ratio of protease:WbdD600 is indicated. (c) Initial WbdD556 crystals (see main text for the crystallization conditions). The dark colour of the crystals arises from the Izit stain (Hampton) that was used to confirm that the crystals are protein. (d) Optimized WbdD556 crystal.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 68| Part 10| October 2012| Pages 1371-1379
https://doi.org/10.1107/S0907444912029599
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