Figure 3
Structure of Deg8 (S292A) in comparison with DegP6 and Deg1 and oligomeric state analysis. (a) Structures of Deg8 (S292A) (green and magenta), DegP6 (PDB entry 1ky9
; red and orange; the second PDZ domain is not shown for clarity; Krojer et al., 2008) and Deg1 (PDB entry 3qo6
; cyan and blue). Protease domains and PDZ domains are shown in cartoon and ribbon modes, respectively. (b) The cavity formed in Deg8, DegP6 (PDB entry 1ky9
; molecule B) and Deg1 (PDB entry 3qo6
). (c) Structural comparison of protomers of Deg8 (S292A) (green) and Deg1 (PDB entry 3qo6
; cyan); the difference in PDZ-domain orientations is indicated. (d) Analytical SEC of Deg8 at pH 6.0 (red line) and pH 8.0 (blue line). Different oligomeric states are labelled above the peaks. |