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Figure 3
Mock-up of what a volume browser for three-dimensional cellular imaging data could look like, using HIV/SIV as an example. The three leftmost images are derived from a 3DSEM reconstruction and show HIV virion reservoirs in infected macrophages (from Bennett et al., 2009BB11; adapted under the terms of the Creative Commons Public Domain declaration). These three orthogonal cross-sections and the cube help users to orient themselves in the data. The central panel shows a slice from a cryo-electron tomographic reconstruction in which the features of individual SIV virus particles can be identified (from Bennett et al., 2007BB10; adapted under the terms for noncommercial use; http://www.jbc.org/site/misc/Copyright_Permission.xhtml ). Here, biologically meaningful segmentations have been overlaid on the tomogram and the corresponding annotations are shown in the top right panel. The bottom right panel shows a three-dimensional rendering of data from EMDB (gold-coloured density from a sub-tomogram average of a HIV viral spike; EMDB entry 5018; Liu et al., 2008BB61) and PDB (the fitted atomic model inside it; structure of a HIV-1 gp120 trimer; PDB entry 3dno ; Liu et al., 2008BB61).

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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