Figure 3
Comparison of flagellar FlhBC and its paralogues from the needle type III secretion system. (a) Multiple sequence alignment of FlhBC from S. typhimurium (SalFlhBC; Swiss-Prot accession No. P40727) with FlhBC from A. aeolicus (AquFlhBC; O67813), EscUC from E. coli (Q7DB59), YscUC from Yersinia pestis (P69986), SpaSC from S. typhimurium (P40702) and Spa40C from Shigella flexneri (Q6XVW1). Identical residues are boxed in red; similar residues are coloured red. Alignment was performed with ClustalW (Larkin et al., 2007; Goujon et al., 2010). (b) Stereoviews of the superposition of Salmonella FlhBC (blue; PDB entry 3b0z
; this work), Aquifex FlhBC (red; PDB entry 3b1s
; this work), EscUC (orange; PDB entry 3bzo
; Zarivach et al., 2008), YscUC (grey; PDB entry 2jli
; Lountos et al., 2009), SpaSC (green; PDB entry 3c01
; Zarivach et al., 2008) and Spa40C (purple; PDB entry 2vt1
; Deane et al., 2008). |