 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 4]](mh5081fig4mag.jpg)
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Figure 4
Effect of mutations of the ENKMS281–285 region of Salmonella FlhB on protein function. (a) Ribbon representation of Salmonella FlhBC; the region is shown in black. (b) The ability of FlhB variants with a modified ENKMS region to complement the ΔflhB Salmonella strain MKM50. Motility assays were carried out on semi-solid agar plates at 303 K for 5 h. FlhB products were 1, none, empty vector; 2, wild-type FlhB; 3, FlhB Δ(281–285); 4, FlhB AAAAA281–285; 5, FlhB PPPPP281–285. (c) Immunoblotting using anti-FlgE and anti-FliC antibodies on the whole-cell (Cell) and culture-supernatant (Sup) fractions from Salmonella strain MKM50 producing different FlhB variants. |
![[Figure 4]](mh5081fig4.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
