Using high-throughput in situ plate screening to evaluate the effect of dehydration on protein crystals

Douangamath, A.; Aller, P.; Lukacik, P.; Sanchez-Weatherby, J.; Moraes, I.; Brandao-Neto, J.

doi:10.1107/S0907444913002412

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 2
Effect of salt concentration on protein unit-cell parameters at 293 K: (a) on the a and b axes and (b) on the c axis. For each salt concentration 2–12 crystals were used for the calculation of statistics. To exclude the statistical outliers, we calculated the mean value ( $[\overline x]$ ) for each unit-cell parameter and the corresponding standard error of the mean, σ: σ = $[\{[\textstyle \sum (x - {\overline x})^2]/n - 1\}^{1/2}]$ /n^1/2, where n is the number of crystals at a single salt concentration. If one of the unit-cell parameters did not follow the rule $[{\overline x} - 3\sigma \,\,\lt \,\,x \,\,\lt \,\,{\overline x} + 3\sigma]$ , the crystal was considered to be an outlier and was discarded from the final calculation of statistics. The error bar shows the standard error of the mean $[({\overline x} \pm 3\sigma)]$ .

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 69| Part 5| April 2013| Pages 920-923

doi:10.1107/S0907444913002412

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