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Figure 1
Efficiencies achieved by careful pre-visit preparation. (a) The distribution of diffraction experiments performed by the SGC throughout 2011 illustrates the value of testing crystals in-house: three quarters of crystals could be discarded as inferior, allowing beamtime to be dedicated to the collection of high-quality data sets, so that half led to deposited structures. (b) A well structured list of priorities such as this example from our laboratory, prepared and distributed in advance of the visit, greatly speeds up experiments by enabling decisions to be made rapidly. (c) A comparison of the best resolutions observed for a series of projects with various beams (see legend) illustrates that diffraction can generally be reliably identified from less intense beams and that improvements may actually be quite modest. (d) The very different improvements in resolution observed for crystals from two different projects using a trimmed and microfocused beam (c) can be rationalized by the ratio of the crystal size to the respective beam sizes: if the crystal is already much larger than the beam (ABL2) an even smaller beam will not help much.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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