 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 2]](ba5199fig2mag.jpg)
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Figure 2
Removal of surface ice with a jet of liquid nitrogen from a BryMill cryosurgery device. The process is efficient and safe, provided that it is performed correctly. (a) In order not to blow away the cold air stream the jet should be at an acute angle to it, and it should not be pointed directly at the crystal but instead swept towards it across the cold air stream. There must be sufficient pressure so the jet does not splutter and large loops should be turned so their edge faces the jet, because the sample drop does not stick well to the loop. (b) A nozzle with a non-uniform edge will produce a divergent jet which may blow the cold stream away and lead to destruction of the sample; a quick remedy is to drill out one of the inserts supplied. (c) Image of the loop before and after deicing. |
![[Figure 2]](ba5199fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
