Figure 1
SDS–PAGE of purified CobDH, which has an apparent molecular mass of 22.5 kDa, in line with its calculated molecular mass. Lane 1, marker (labelled in kDa); lane 2, after Ni–NTA; lanes 3–5, after gel filtration. Purified CobDH is indicated by an asterisk. Protein bands were stained with InstantBlue dye (Expedeon). |