Figure 2
Sequence alignment of procaspase-7 variants. I, procaspase-7 devoid of the N-terminal 46 amino acids (Δ46 procaspase-7); II, amino acids 195–200 of Δ46 procaspase-7 were replaced with LVPRGS (a sequence susceptible to thrombin) with the additional deletion of PINDT from the L2 loop; III, amino acids 195–200 of Δ46 procaspase-7 were replaced by LVPRGS; IV, an additional LVPRGS motif was inserted between Asp204 and Thr205 of Δ46 procaspase-7 and Asp198 was mutated to Ala. |