Combining crystallography and EPR: crystal and solution structures of the multidomain cochaperone DnaJ

Barends, T.R.M.; Brosi, R.W.W.; Steinmetz, A.; Scherer, A.; Hartmann, E.; Eschenbach, J.; Lorenz, T.; Seidel, R.; Shoeman, R.L.; Zimmermann, S.; Bittl, R.; Schlichting, I.; Reinstein, J.

doi:10.1107/S0907444913010640

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 5
(a–c) X-band pELDOR measurements of DnaJ double mutants (a) T18C/S86C, (b) S86C/E95C and (c) I50C/V90C: background-corrected time traces and their Tikhonov regularization fits (left; regularization parameter α = 10) and corresponding distance distributions superimposed on their respective rotamer distribution predicted from the crystal structure (right). (d) X-band pELDOR measurements of DnaJ single mutants S86C (black) and S58C (blue) using a long time window for measurements of large distances. Dipolar evolution functions with Tikhonov regularization fit (inset; α = 100) and corresponding distance distributions (right). Colour codes above the distributions signify reliability intervals; the reliability of the mean distance of the calculated distribution peaks is shown from dark blue (highest reliability) to red (lowest reliability).

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 69| Part 8| July 2013| Pages 1540-1552

doi:10.1107/S0907444913010640

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