Figure 4
Diffraction images of bacterial hydrolase RsbQ crystals. (a) A diffraction image from a crystal treated using a typical cryoprotection procedure. The crystal was mounted with a cryosolution composed of the reservoir solution supplemented with 25%(v/v) glycerol and then flash-cooled. (b) A diffraction image from a crystal mounted with the HAG method. The crystal was mounted using 13%(w/w) PVA3500 glue with a humidity of 69.1% RH and then flash-cooled. Both samples were kept at 100 K. |