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![[Figure 5]](tz5035fig5mag.jpg)
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Figure 5
Reversible crystal lattice transformation of glue-coated lysozyme crystals subjected to humidity changes. (a–c) The effects observed on the crystallographic a axis (top), c axis (middle) and mosaicity (bottom) caused by humidity changes. (a) The response to a reduction in humidity. The filled circles (solid line) indicate continuous measurements of a crystal treated with humid air starting at 87.7% RH and gradually decreasing to 67.6% RH. (b, c) The response to two-way changes in humidity. The lattice-parameter changes during the humidity-reduction process are plotted as filled circles. (b) Humidity reduction from 88.3 to 67.9% RH. (c) Humidity reduction from 87.1 to 69.8% RH. In (b) and (c) the triangles (dotted line) indicate the trace of lattice-parameter changes during the humidity-recovery process up to 92.5% RH (b) and 90.5% RH (c) after the previous humidity reduction. In addition, the lattice constants observed after flash-cooling are overlaid on (b). For flash-cooling, five new crystals were prepared at five different humidities (74.3, 79.5, 82.7, 87.3 and 88.6% RH). Parameters obtained from the experiments conducted at room temperature and 100 K are plotted as open and filled squares, respectively. (d) Diffraction images collected at the humidity conditions indicated by the four wedges shown in (a). |
![[Figure 5]](tz5035fig5.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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