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![[Figure 7]](tz5035fig7mag.jpg)
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Figure 7
Long-term stability accompanied by reproducible lattice-constant transitions of glue-coated lysozyme crystals treated with consecutive dehydration, rehydration and re-dehydration processes. (a) Time course of the transformation of a crystal subjected to the following programmed humidity changes. In the first stage of the program, the relative humidity was kept at 87.4% RH for 10 min after the lysozyme crystal had been mounted. Next, dehydration (87.4 to 70.0% RH) and subsequent hydration (70.0 to 90.1% RH) experiments were conducted with a stepwise humidity change at a rate of 2.5% RH per 5 min. Finally, re-dehydration (90.1 to 70.3% RH) was applied with a humidity-change rate of 5% RH per 10 min. (b) The reversible changes in the lattice constants in response to humidity changes. The relative changes of the crystallographic a axis (Δa/a) and c axis (Δc/c) and of the unit-cell volume (ΔV/V) are plotted with solid, dotted and grey bold lines, respectively. |
![[Figure 7]](tz5035fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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