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Figure 1
Crystal structure of the AvrBs3–DNA complex. (a) Sketch of the TALE domain structure. The N-terminus is coloured green and the C-terminus is coloured blue. The central DNA-binding domain contains the RVDs involved in DNA recognition. The sequence of the first repeat (cyan) of AvrBs3 is shown, indicating the position of each amino acid in the repeat. The sequence of the oligonucleotide used in crystallization and the different dipeptides is depicted below. (b) Cartoon representation of the protein–DNA structure perpendicular to the DNA target (upper panel) and along the DNA helix (lower panel). The helices of AvrBs3 are shown as cylinders and repeats −1, 0, 1, 2 and 3 are coloured red, lime, cyan, green and beige, respectively, to indicate the initial DNA domain building blocks. The duplex oligonucleotide is represented in stick mode and the sense-strand sequence is indicated with the corresponding dipeptide for each nucleotide. The dipeptides are represented using the single-letter amino-acid code. (c) Electrostatic surface representation of the AvrBs3 protein in complex with its DNA target. The upper panel depicts the electropositive strip (blue) on the superhelical arrangement running from the amino-terminus to the carboxyl-terminus. The electronegative strip (red) running along the protein in the opposite side is depicted in the lower panel. The sense strand is coloured magenta and the antisense strand is coloured green.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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