 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
journal menu
![[Figure 2]](ba5198fig2mag.jpg)
|
|
Figure 2
The low sensitivity of MR searches to nucleic acid sequence conservation. Group II intron structures were derived from the O. iheyensis group II intron structure (PDB entry 3igi ) using (a) the original intron sequence (green tones), (b) a randomly generated sequence (blue tones) or (c) an `opposite' sequence (gray tones). For each sequence, the backbone was distorted using FRODA with increasing values of the r.m.s.d. (from 0 Å in the darkest color to 4 Å in the lightest color). The TFZ scores from Phaser are plotted for each MR run. A TFZ value of 8 or higher was observed to confidently indicate correct solutions for protein structures (McCoy, 2007  ) and is thus taken here as a cutoff level for successful solutions. Although such a TFZ scale may not necessarily apply exactly to nucleic acid structures, we observed that our solutions with TFZ > 8 were generally associated with interpretable electron-density maps. |
![[Figure 2]](ba5198fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
