Structural basis of SUFU–GLI interaction in human Hedgehog signalling regulation

Cherry, A.L.; Finta, C.; Karlström, M.; Jin, Q.; Schwend, T.; Astorga-Wells, J.; Zubarev, R.A.; Del Campo, M.; Criswell, A.R.; de Sanctis, D.; Jovine, L.; Toftgård, R.

doi:10.1107/S0907444913028473

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 7
MBP-SUFU and GLI peptides interact in the same way in solution as in crystals. (a) HDX analysis of MBP-SUFU-FL with GLI3_p (blue). Areas that are more protected from exchange in the presence of the peptide are highlighted in pink. (b) Comparison of SAXS envelopes for MBP-SUFU-Δ in the absence (left) and presence (right) of GLI1_p. Crystal structures of apo MBP-SUFU-Δ and of MBP_{A216H_K220H}-SUFU-Δ_{W61D_L62S_G63F_P453A_Δ454–456_K457A} co-crystallized with GLI3_p superpose well on apo and holo SAXS envelopes, respectively.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 69| Part 12| December 2013| Pages 2563-2579

https://doi.org/10.1107/S0907444913028473

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