Insights into the mechanism of X-ray-induced disulfide-bond cleavage in lysozyme crystals based on EPR, optical absorption and X-ray diffraction studies

Sutton, K.A.; Black, P.J.; Mercer, K.R.; Garman, E.F.; Owen, R.L.; Snell, E.H.; Bernhard, W.A.

doi:10.1107/S0907444913022117

Acta Crystallographica Section D
Acta Crystallographica
Section DSTRUCTURAL BIOLOGY

IUCr IT WDC

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Figure 3
Absorbance measured for (a) 20 × 1 s burns and (b) a single 20 s burn. The absorbed dose per 1 s exposure was 287 kGy. The cumulative dose over 20 s was thus 5.74 MGy. (a) The multiple burns show a progressively smaller change in absorption for the same absorbed dose and rapid loss of $[{\rm SS}^\bullet{^-}]$ was observed after each pulse. (b) The single continuous 20 s burn highlights the post-exposure decay of the disulfide peak, which is best described by a two-rate model, in agreement with previous observations (Owen et al., 2011