Figure 4
(a) OMIT difference 2Fo − Fc electron density of xylohexaose in E177Q–X6 contoured at the 1.0σ level. (b) Chemical diagram of conventional direct hydrogen-bond interactions between X6 and E177Q residues. (c) Water-mediated interactions between X6 and the active-site residues and the unconventional C—H⋯O hydrogen bonds between Glu86 and the −1 xylose subunit in E177Q–X6. |