 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 4]](dw5063fig4mag.jpg)
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Figure 4
MT pelleting assays in the presence of Kif15 motor or tail domains. (a) MT pelleting assays of the Kif15 motor domain in the presence of various nucleotides. Increasing amounts of Kif1519–375 (1–12 µM) were incubated with MTs (5 µM) in the presence of 2 mM Mg2+-ATP, 2 mM AMP-PNP or 4 mU apyrase. Samples of supernatants and pellets were analysed by SDS–PAGE. (b) MT binding of Kif1519–375 in the presence of 2 mM Mg2+-ATP, 2 mM AMP-PNP or 4 mU apyrase. The plotted data relate to the amounts (µM) of Kif1519–375 recovered from supernatant and pellet (pelleted with MTs) fractions of reactions run in the presence of various nucleotides. Data were obtained by analysing the SDS–PAGE (ImageJ 143.u) presented in (a). (c) MT pelleting assays of the Kif15 tail domain. Increasing amounts of Kif151149–1388 (1–10 µM) were incubated with MTs (5 µM). Samples of supernatants and pellets were analysed by SDS–PAGE. The plotted data relate to the amounts (µM) of Kif151149–1388 recovered from supernatant and pellet (pelleted with MTs) fractions. Data were obtained by analysing the SDS–PAGE (ImageJ 143.u) presented on the left side of the figure. |
![[Figure 4]](dw5063fig4.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
