Figure 2
Superposition of the active sites of the catalytic domains of astacin and NleC. (a) The superposed active sites of astacin and NleC. The astacin catalytic domain is shown in marine and that of NleC in magenta. (b) The active site of NleC. (c) The active site of the catalytic domain of astacin. (d) NleC shown to be a zincin protease. The mutation of any of the zinc-binding ligands disrupts the protease activity of NleC (lanes 5–8), with the exception of the fifth ligand Tyr227. The Y227A mutant crippled the protease activity (lane 4). |