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Figure 4
Electrostatic surface potentials of NleC and p65. (a) Surface potential of NleC. The mutated negatively charged glutamate residues are labelled. (b) Surface potential of p65. The DNA-binding loop cleaved by NleC is indicated by a green dashed circle. (c) Surface potential of NleC with the superposed reaction-intermediate analogue from astacin (PDB entry 1qji ). (d) Cartoon representation of p65. The scissile bond between Cys38 and Glu39 is depicted in magenta. (e) Mutation of the negatively charged residues on the surface of NleC abolished the metalloprotease activity.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 1| December 2014| Pages 40-47
doi:10.1107/S1399004713024619
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