Figure 1
Structure of proCASP6H121A. (a) The overall structure of proCASP6H121A. (b) Structure overlay of monomer A of proCASP6H121A and the ΔproCASP6 zymogen. (c) Active site of monomer A. (d) Active-site overlay of monomer A of proCASP6H121A and the ΔproCASP6 zymogen. (e) Structure overlay of monomer B of proCASP6H121A and p20/p10S257E. (f) Interaction between monomer A and the neighbouring molecule in the proCASP6H121A crystal. (g) The clash between two well formed active sites of the ΔproCASP6 zymogen when superimposed on two symmetric B monomers of the proCASP6H121A crystal. The electron-density map (2Fo − Fc maps) is shown at 1.0σ and was calculated by phenix.refine. Hydrogen bonds are shown as blue dashed lines. |