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Figure 4
The priority of the TETD23 site over the TEVD193 site during intermolecular cleavage was not caused by the primary sequence. (a, b) Coomassie Blue-stained SDS–PAGE of (a) proCASP6(C163A,RE) and (b) proCASP6(T22V,V192T,C163A,RE) incubated with 1% active CASP6 for 30 min. (c) Activity assay of active CASP6 with different substrates. The concentration of the substrates was 50 µM and the concentration of active CASP6 was about 16.7 nM. The activities were normalized to the substrate Ac-TETD-AFC. Assays were performed in triplicate and error bars represent standard deviations. RE, R(64,220)E; FL, full length; p20, large subunit; L, intersubunit linker; p10, small subunit.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 1| December 2014| Pages 58-67
doi:10.1107/S1399004713024218
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